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grna screening vector  (Addgene inc)


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    Structured Review

    Addgene inc grna screening vector
    a , Overview of <t>gRNA</t> assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a <t>non-functional</t> <t>EGFP</t> that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Grna Screening Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+screening+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/pmc12510883-351-1-9
    Average 96 stars, based on 124 article reviews
    grna screening vector - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing"

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing

    Journal: Nature Methods

    doi: 10.1038/s41592-025-02805-0

    a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Figure Legend Snippet: a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Techniques Used: Binding Assay, Functional Assay, Flow Cytometry

    Related Articles

    Plasmid Preparation:

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA-RNA sequencing.
    Article Snippet: The BE vectors were all-in-one cytosine or adenine base editor + guide expression constructs (Addgene, 158581 and 179097). .. The gRNA screening vector was a modified CROP-seq vector (Addgene, 86708) to also express eGFP and include a distinct gRNA CS in the scaffold of the gRNA76. ..

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
    Article Snippet: The BE vectors were all-in-one cytosine or adenine base editor + guide expression constructs (Addgene, 158581 and 179097). .. The gRNA screening vector was a modified CROP-seq vector (Addgene, 86708) to also express eGFP and include a distinct gRNA CS in the scaffold of the gRNA . ..

    Modification:

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA-RNA sequencing.
    Article Snippet: The BE vectors were all-in-one cytosine or adenine base editor + guide expression constructs (Addgene, 158581 and 179097). .. The gRNA screening vector was a modified CROP-seq vector (Addgene, 86708) to also express eGFP and include a distinct gRNA CS in the scaffold of the gRNA76. ..

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
    Article Snippet: The BE vectors were all-in-one cytosine or adenine base editor + guide expression constructs (Addgene, 158581 and 179097). .. The gRNA screening vector was a modified CROP-seq vector (Addgene, 86708) to also express eGFP and include a distinct gRNA CS in the scaffold of the gRNA . ..



    Similar Products

    grna screening vector  (Addgene inc)
    96
    Addgene inc grna screening vector
    a , Overview of <t>gRNA</t> assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a <t>non-functional</t> <t>EGFP</t> that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Grna Screening Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+screening+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/pmc12510883-351-1-9
    Average 96 stars, based on 1 article reviews
    grna screening vector - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Journal: Nature Methods

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing

    doi: 10.1038/s41592-025-02805-0

    Figure Lengend Snippet: a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Article Snippet: The gRNA screening vector was a modified CROP-seq vector (Addgene, 86708) to also express eGFP and include a distinct gRNA CS in the scaffold of the gRNA .

    Techniques: Binding Assay, Functional Assay, Flow Cytometry